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Amersham Life Sciences Inc high stringency hybridization buffer
High Stringency Hybridization Buffer, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+stringency+hybridization+buffer/high+stringency+hybridization+buffer/pm16685698-43-19-23
Average 90 stars, based on 1 article reviews
high stringency hybridization buffer - by Bioz Stars, 2026-09
90/100 stars

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Hybridization:

Article Title: Telomere length predicts poststroke mortality, dementia, and cognitive decline.
Article Snippet: Objective: Long-term cognitive development is variable among stroke survivors, with a high proportion developing dementia.. Early identification of those at risk is highly desirable to target interventions for secondary prevention.. Telomere length in peripheral blood mononuclear cells was tested as prognostic risk marker.

Article Title: Telomere shortening in human fibroblasts is not dependent on the size of the telomeric-3'-overhang.
Article Snippet: .. The length of single-stranded terminal overhangs in telomeres was measured by in-gel hybridization of telomeric probes onto nondenatured DNA as described (van Steensel et al ., 1998; von Zglinicki et al ., 2000a) with the following modifications: A 1% agarose gel was dried at room temperature and the nondenatured gel was hybridized with 32 Pγ -ATP end-labelled (CCCTAA) 4 or, as control (TTAGGG) 4 at 30 °C for 16 h using high stringency hybridization buffer (Amersham, Chalfont St. Giles, Bucks, UK). .. Gels were washed 4 times in 0.2 × SSC at room temperature for 30 min each and then exposed overnight in a phosphoimager (Storm 820, Molecular Dynamics, Amersham).

Agarose Gel Electrophoresis:

Article Title: Telomere shortening in human fibroblasts is not dependent on the size of the telomeric-3'-overhang.
Article Snippet: .. The length of single-stranded terminal overhangs in telomeres was measured by in-gel hybridization of telomeric probes onto nondenatured DNA as described (van Steensel et al ., 1998; von Zglinicki et al ., 2000a) with the following modifications: A 1% agarose gel was dried at room temperature and the nondenatured gel was hybridized with 32 Pγ -ATP end-labelled (CCCTAA) 4 or, as control (TTAGGG) 4 at 30 °C for 16 h using high stringency hybridization buffer (Amersham, Chalfont St. Giles, Bucks, UK). .. Gels were washed 4 times in 0.2 × SSC at room temperature for 30 min each and then exposed overnight in a phosphoimager (Storm 820, Molecular Dynamics, Amersham).

Control:

Article Title: Telomere shortening in human fibroblasts is not dependent on the size of the telomeric-3'-overhang.
Article Snippet: .. The length of single-stranded terminal overhangs in telomeres was measured by in-gel hybridization of telomeric probes onto nondenatured DNA as described (van Steensel et al ., 1998; von Zglinicki et al ., 2000a) with the following modifications: A 1% agarose gel was dried at room temperature and the nondenatured gel was hybridized with 32 Pγ -ATP end-labelled (CCCTAA) 4 or, as control (TTAGGG) 4 at 30 °C for 16 h using high stringency hybridization buffer (Amersham, Chalfont St. Giles, Bucks, UK). .. Gels were washed 4 times in 0.2 × SSC at room temperature for 30 min each and then exposed overnight in a phosphoimager (Storm 820, Molecular Dynamics, Amersham).



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